Membrane Protein Stabilization Strategies for Structural and Functional Studies
نویسندگان
چکیده
منابع مشابه
Functional and Structural Studies on Heptahelical Membrane Proteins
12 Lyhennelmä (Synopsis in Finnish) 13
متن کاملMembrane protein stabilization
sequences is therefore important for characterizing immune responses, but it cannot describe the state of cells within a given clone. Teichmann and colleagues present TraCeR, a tool that allows TCR sequences to be reconstructed from single-cell RNA sequencing data from T cells. The approach combines immune profiling with information on fate and function based on the expression of thousands of g...
متن کاملTandem facial amphiphiles for membrane protein stabilization.
We describe a new type of synthetic amphiphile that is intended to support biochemical characterization of intrinsic membrane proteins. Members of this new family displayed favorable behavior with four of five membrane proteins tested, and these amphiphiles formed relatively small micelles.
متن کاملCurrent strategies for protein production and purification enabling membrane protein structural biology.
Membrane proteins are still heavily under-represented in the protein data bank (PDB), owing to multiple bottlenecks. The typical low abundance of membrane proteins in their natural hosts makes it necessary to overexpress these proteins either in heterologous systems or through in vitro translation/cell-free expression. Heterologous expression of proteins, in turn, leads to multiple obstacles, o...
متن کاملStructural and functional studies of complement inhibitor C4b-binding protein.
C4b-binding protein (C4BP) is a potent inhibitor of the classical pathway of the complement system. This large plasma glycoprotein consists of seven identical alpha-chains and a unique beta-chain held together by disulphide bridges. Both types of subunits are composed almost exclusively of complement control protein domains (CCPs). Using homology-based computer modelling and mutagenesis of reco...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Membranes
سال: 2021
ISSN: 2077-0375
DOI: 10.3390/membranes11020155